Author: ROMUALD EIMONT
Dissertation title: Synthesis and nanofabrication of biomimetic hydrogels
Fields of science: Chemistry N 003
Scientific supervisor: dr. Ramūnas Valiokas
Defence of the dissertation: 16 May 2025
SUMMARY: The goal of tissue engineering is to restore the functions of damaged tissues or organs using bioimitative materials. One of the most crucial components in this process is the extracellular matrix, whose physical and mechanical properties significantly influence cell behavior, regeneration, and differentiation. The development and application of materials that mimic the extracellular matrix are essential to the tissue engineering process. This dissertation explores the formation and modification possibilities of collagen-based hydrogels and their application in organotypic cell cultures. Micro- and nanostructured surfaces were created and analyzed, evaluating their effects on skin fibroblasts. Additionally, an in vitro corneal model suitable for research was developed. The influence of hydrogels on cardiomyocyte differentiation was examined, as well as their suitability for cultivating neural cells and cerebellar organoids. Finally, modified peptide hydrogels were created, incorporating different biologically active amino acid sequences. The results of this dissertation contribute to the development of advanced in vitro tissue modeling technologies.